Uji Aktivitas Antioksidan Ekstrak Metanol dan Fraksi Kulit Buah Markisa Konyal (Passiflora Ligularis Juss) dengan Metoda DPPH: Penelitian

Meli Oktavia; Kharisma Jayak Pratama; Niken Luthfiyanti

doi:10.31004/jerkin.v3i4.845

Authors

Meli Oktavia Universitas Duta Bangsa Surakarta
Kharisma Jayak Pratama Universitas Duta Bangsa Surakarta
Niken Luthfiyanti Universitas Duta Bangsa Surakarta

DOI:

https://doi.org/10.31004/jerkin.v3i4.845

Keywords:

Antioksidan, DPPH, Ekstraksi, Markisa Konyal

Abstract

Antioxidants are compounds that can absorb or neutralize free radicals so that they can ward pff disease. Free radicals are foreign compounds that enter the body and damage the body’s immune system. Konyal passion fruit seeds (Passiflora ligularis Juss) have been widely used as passion fruit juice. Konyal passion fruit skin is a waste that can be used as a natural medicine. This study aims to determine the antioxidant activity of methanol extract and passion fruit skin fraction (Passiflora ligularis Juss) with the DPPH method using UV-VIS spectrophotometry at a maximum absorption wavelength of 517 nm with vitamin C as a comparator. The antioxidant activity test obtained IC₅₀ Vitamin C : 3,054 µg/mL, IC₅₀ methanol extract : 222,5 µg/mL, IC₅₀ n-hexane fraction : 93,71 µg/mL, IC₅₀ ethyl acetate fraction : 205,29 µg/mL and IC₅₀ water fraction 124,76 µg/mL. Based on the IC₅₀value, it can be concluded that the antioxidant activity of the methanol extract is in the weak category, the n-heksan fraction is in te strong category, the ethyl acetate fraction is in the weak category and the water fraction is in the medium category.

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