Effectiveness Test of The Combined Extract of Papaya Seeds (Carica Papaya L.) and Sweet Orange Peel (Citrus Sinesis L.) as A Larvicide Against Aedes Aegypti Mosquitoes

Penelitian

Abstract

Dengue Hemorrhagic Fever (DHF) remains one of the most significant public health problems in Indonesia. This study aims to determine the effectiveness of a mixture of papaya seed (Carica papaya L.) and sweet orange peel (Citrus sinensis L.) extracts as a botanical larvicide against Aedes aegypti mosquito larvae. A quasi-experimental study was conducted using a Completely Randomized Design with five treatments and four replications. Three concentrations (1%, 1.5%, and 2%) of the mixed extract were tested with observation for 12 hours. The mortality rate of larvae increased with the concentration: 1% (30%), 1.5% (67.5%), and 2% (100%). ANOVA and Duncan tests confirmed significant differences (p<0.05). The synergistic action of flavonoids, saponins, tannins, papain, and limonoids enhanced larval mortality, indicating the potential of plant-based larvicides as an eco-friendly alternative to synthetic chemicals.